Antisera for tissue-typing: the production of cytotoxic and agglutinating antibodies following intradermal leukocyte injections in man.

L EUKOCYTE TYPING may prove to be an important guide in determining histocompatibility relationships between donor and recipient in tissue l 2 Most studies directed towards ascertaining correlations between human leukocyte factors are carried out with batteries of multispecific antisera. The reactions of such antisera tend, unfortunately, to mask antigenic differences between closely linked factors.3 Monospecific antisera are needed for the reliable delineation of these factors, as well as for genetic studies of histocompatibility antigens.4’5 The present work was undertaken in an attempt to produce monospecffic antisera by immunization of recipients with leukocytes from closely matched partners.